Aim
To identify the parts of an embryo of a dicot seed (gram/pea).
Materials Required
Soaked gram/pea seeds
Forceps
Needle
Hand lens
Water
Petri dish
Procedure
- Take a few gram or pea seeds and soak them in water overnight.
- Drain the water and gently press a seed between your fingers to see the micropyle (small pore).
- Carefully remove the thin outer covering called the seed coat (testa) using forceps.
- Gently open the two halves of the seed, which are the cotyledons.
- Observe the small structure attached to the cotyledons, which is the embryo.
- Identify the radicle (at the bottom), plumule (at the top), and the embryonal axis connecting them.
Precautions
- Soak the seeds for at least 12 hours so they become soft and easy to dissect.
- Handle the seeds gently to avoid breaking the delicate embryo.
- Use a hand lens to carefully observe small parts like the radicle and plumule.
Dissection Controls
Step through the dissection of a soaked gram seed.
Status: Whole seed with seed coat intact.
Compare: Dicot vs Monocot Seed
Dicot (e.g., Gram/Pea): Has TWO cotyledons. Food is stored in cotyledons. Endosperm is usually absent in mature seeds.
Monocot (e.g., Maize/Wheat): Has ONE cotyledon (scutellum). Food is mostly stored in the endosperm.
Observation Table
| S.No. | Part of Seed | Description | Function |
|---|
Experiment Video
Assessment
Lab Report
Aim: To identify the parts of an embryo of a dicot seed (gram/pea).
Observations:
- No observations recorded yet. Complete the simulation.
Result: We identified the main parts of a dicot seed: seed coat (testa), two cotyledons, radicle, plumule, and embryonal axis.